Biotechnological analytics

Course contents
  • Overview of chromatographic methods
    - Structure of HPLC: components and working principle
    - Theoretical basis: retention factor, resolution, theoretical plates, van Deemter, mass transfer, symmetry, peak widening
    - Separation principles: IEC, ALC, SEC, NP, RP, HIC, HILIC, IP-RP
    - Stationary phases, functional groups, separation mechanisms, eluent composition
    - HPLC optimization, troubleshooting and miniaturization
    - Optimization of isocratic and gradient separation, micro- and nano-HPLC
    - Other separation methods: CE, SDS-PAGE, ELISA, IEF (fundamentals of separation mechanisms and fields of application)
    - MS: ionization techniques (ESI, EI, MALDI, APCI, APPI, FAB), mass analyzers (single/triple quadrupole, 2D/3D ion trap, TOF, ICR, hybrids (qTOF, orbitrap)
    - Hyphenated techniques: HPLC-MS (industrial application to biomolecules (proteins, DNA))