Project Biotechnology 3

Course contents
  • This course is a continuation to the practical course "Project Biotechnology 2". The recombinant Pichia pastoris strains established in the previous project parts will be fermented in a large scale during this course. This includes:
    - planning the fermentation
    - preparation of media and solutions
    - prearrangement of fermenter: calibrations, filling, sterilization
    - preparation of preculture and culture
    - fermentation: inoculation, sterile sample taking, data logging, induction of protein expression, batch and fed-batch fermentation
    - analytics
    - evaluation of results:
    comparison of growth behavior of native and recombinant strain
    comparison of growth behavior in shaking flasks and fermentor
    - functional assay for the expressed proteins