Project Biotechnology 3

Course contents
This course is a continuation to the practical course "Project Biotechnology 2". The recombinant Pichia pastoris strains established in the previous project parts will be fermented in a large scale during this course. This includes:
- planning the fermentation
- preparation of media and solutions
- prearrangement of fermenter: calibrations, filling, sterilization
- preparation of preculture and culture
- fermentation: inoculation, sterile sample taking, data logging, induction of protein expression, batch and fed-batch fermentation
- analytics
- evaluation of results:
comparison of growth behavior of native and recombinant strain
comparison of growth behavior in shaking flasks and fermentor
- functional assay for the expressed proteins